DAISO CO., LTD. | DAISO SILICA GEL


SP-120-BIO SP-200-BIO SP-300-BIO


SP-100-P SP-100-ODS-P


ODS-BP ODS-AP C8-P C4-P C1-P APS-P SP-P

	SP-1000 SP-2000


Packing services Empty columns BIO-columns HSA-columns Standard columns

Suitable for hydrophilic compounds separation
Strong retention in aqueous condition
Longer lifetime in aqueous eluents
Different selectivity from ODS-AP
Enhanced mechanical stability
Suitable for Dynamic Axial Compression columns

ODS-BP phases are designed to show extended selectivity for hydrophilic and polar compounds which are either not or poorly retained on other phases. A proprietary modification technique avoids the matting-down effect of the C18 chains which conventional ODS-phases show at high water contents in the mobile phase, even if pure water is used. Typical applications are separations of biomolecules and metabolites such as oligosaccharides, amino acids, small peptides, nucleotides and organic acids.
DAISOGEL ODS-BP phases are fully endcapped and show similar selectivity as conventional C18 phases when being used for separations of hydrophobic compounds with typical reversed phase eluents.
DAISOGEL ODS-BP phases show stable base lines and high sensitivity even under neutral pH conditions and without buffer or counter-ion additives, which makes them appear especially suited for hyphenated techniques like LC-MS, where such additives disturb the detection.

Product names and properties / analytical grades (minimum lot: 50g)

	Pore Size (nm)	Particle Size (um)	Pore Volume (mL/g)	Surface Area (m²/g)	% of Carbon
SP-120-3-ODS-BP	12	3	1	300	15
SP-120-4-ODS-BP	12	4	1	300	15
SP-120-5-ODS-BP	12	5	1	300	15
SP-120-7-ODS-BP	12	7	1	300	15
SP-200-3-ODS-BP	20	3	1.1	200	10
SP-200-5-ODS-BP	20	5	1.1	200	10

Product names and properties / preparative grades (minimum lot: 500g)

	Pore Size (nm)	Particle Size (um)	Pore Volume (mL/g)	Surface Area (m²/g)	% of Carbon
SP-120-10-ODS-BP	12	10	1	300	15
SP-120-15-ODS-BP	12	15	1	300	15
SP-120-20-ODS-BP	12	20	1	300	15
SP-120-40/60-ODS-B	12	50	1	300	15
SP-200-10-ODS-BP	20	10	1.1	200	10
SP-200-15-ODS-BP	20	15	1.1	200	10
SP-200-20-ODS-BP	20	20	1.1	200	10
SP-200-40/60-ODS-B	20	50	1.1	200	10

Alanine and its oligopeptides are separated on DAISOGEL ODS-BP using 100% water as eluent. The elution sequence corresponds with the number of amino acid units included in the each peptide.
The diastereoisomer which contains the unnatural D-Ala in its structure shows a different retention time from the corresponding all-L-Ala peptide with the same number of amino acid residue.

Peptides were eluted on
DAISOGEL SP-120-5-ODS-BP (6.0 x 150mm) with H2O
Flow rate, 1ml/min. Detector : UV214nm.

1-Kestose, 6-Kestose and Neokestose are position diastereoisomers which have the same molecular weight and are built up by the same monosaccharides, but they differ in the bonding position between sucrose and fructose. ODS-BP is sensitive to such small differences.

Disaccharides such as cellobiose and sucrose can be efficiently separated by ODS-BP. These disaccharides are composed of different monosaccharide units and exhibit different hydrophobicity. ODS-BP is capable of recognizing such a small differences.

Intensity of hydrophobic interaction of ODS-BP

The figure shows the intensity of interaction between solutes and ODS-BP.
The x-axis shows the number of carbon atoms of the solute, the y-axis shows log K‘, which is calculated from the retention time of each solute. The fact that there is a linear relationship between these parameters proves that the retention mechanism of ODS-BP is based on van der Waals forces. Every side-chain added changes the hydrophobicity of the solute and influences the retention behavior.

	0 hours	after 100 hours
Pyridine kpy	0.704	0.686
Phenol kph	2.129	2.080
Separation α	3.024	3.032

There is no evidence of phase collapse of DAISOGEL ODS-BP with pure water. The test chromatogram of the pyridine/phenol separations shows that after 100 hours washing with water there was no change in selectivity or retention behavior.